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In Vitro Comparative Study of Antioxidant and Antibacterial Activity of Selected Dietary Plants
Palvi Sharma
Nepal Journal of Biotechnology
Ethanolic extracts of Garlic (Bulb), Aloe (leaf), Flower bud (buds), Turmeric (rhizomes) and Ginger (rhizomes) were used for relative analysis of antioxidant and antimicrobial activity. Antioxidant activity was determined by DPPH [1, 1-Diphenyl-2-picryl hydrazyl] assay and expressed with Ascorbic acid. It was observed that turmeric and ginger have more antioxidant activity than garlic, Aloe and Flower bud. These extracts were further studied for antibacterial activity by agar well diffusion and spectrophotometric method against tetracycline as reference. The result showed that Flower bud is more effective against Escherichia coli, Pseudomonas aeuroginosa, Bacillus subtilis and Staphylococcus aureus compared to other plants extract. However, all the plants extract did show antioxidant and antibacterial activity.
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Evaluation of Prebiotic and Antimicrobial Properties of Natural Extracts
Naina Bhanjer, International Journal of Scientific Research in Science and Technology IJSRST
International Journal of Scientific Research in Science and Technology, 2021
Prebiotics are compounds in food that induce growth or activity of beneficial microorganisms & are beneficial for host health. The most common example is in the gastrointestinal tract, where prebiotics alter composition of organisms in the gut microbiome. Prebiotics are the non-digestible fibers or carbohydrates that gut bacteria can digest, and prebiotics adds nutritional value & help healthy bacteria grow. Prebiotics are associated with Probiotic, microbes which keep intestine healthy and enhance immune system by fighting with harmful bacteria. This study centers on evaluation of prebiotic properties for natural, aqueous extracts of Aloe vera (Aloe barbadensis miller), Kokum dried (Garcinia indica), and Wheatgrass (Triticum aestivum) by stimulation of growth, of probiotic organism Lactobacillus casei isolated on Sterile Man Rogosa Sharpe Agar and was measured colorimetrically at 545 nm. Antimicrobial and biopreservative study were also performed of these extracts. Hence to evaluate the variable properties of being used in cancer treatment, cosmetics, food industry, etc. Presence of Vitamin C in these plants by DCPIP method broadens the idea of Prebiotics applications, like better immune health and wound healing. Probiotic organism is also used in horticulture, in this study it resulted in enhancing the process of germination, Lactobacillus casei of 108cfu/ml was incorporated in soil to enhance germination of fenugreek seed and kokum extract as a prebiotic source. Additionally, an online survey with Questionnaire methodology was conducted on Awareness and usage about prebiotic and probiotics by online platform and results were analyzed by graphical and statistical method.
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Antioxidant and Antimicrobial Activity of Edible Plants and Their Potential Use as Nutraceuticals
Daniela Barros de Oliveira
International Symposium on Medicinal and Nutraceutical Plants, 2007
Present work reports a comparative account of chemical composition, antioxidant and antimicrobial activity in the supernatants of Anacardium occidentale L. (cashew) fruits, Xanthosoma sagittifolium (L.) Schott. (cocoyam) cormel, Malpighia glabra L. (acerola) fruits, and Rosmarinus officinalis leaves obtained by ethanol precipitation of the aqueous extracts. Samples were evaluated as radical scavengers by DPPH radical scavenging method. HPLC-DAD chromatograms were performed to identify the flavonoids and broth dilution method to determine the antimicrobial activity of plant extracts. A. occidentale and M. glabra showed DPPH radical scavenging activity above 90% at 100 µg/ml. A. occidentale kept its activity at about 50% at 10 µg/ml. The content of flavonoids can influence the antioxidant activity as it was the case for A. occidentale and X. sagittifolium. In the cases of M. glabra the antioxidant activity maybe due to its content of vitamin C and anthocyanins not detected under our HPLC-DAD conditions. The flavonoid content of A. occidentale and X. sagittifolium influenced antimicrobial activity, but flavonoids in M. glabra and R. officinalis had no antimicrobial activity.
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Antioxidant Activity and Growth Influence of Lactobacillus bulgaricus by Selected Herbal Extracts
BALASUBRAMANIAN SATHYAMURTHY
Int.J.Curr.Microbiol.App.Sci, 2014
Antioxidant capacity of selected herbal extracts and Lactobacillus bulgaricus were studied with DPPH assay. The growth pattern analysis was done for Lactobacillus bulgaricus individually and with herbal extracts. Based on the different reaction mechanisms such as (herbal extract and DPPH), (herbal extract, DPPH andH2O2), (herbal extract, DPPH, H2O2 and culture), (herbal extract, DPPH and culture) the inhibition percentage values for each of these reaction mechanism were calculated and the ANOVA Single Factor test is done and tabulated. The P value obtained for herbal extract + DPPH% inhibition and herbal extract, DPPH, H2O2 % inhibition values are significant. It was concluded that out of the five herbal extracts Ocium sanctum (Tulsi) is said to be significant suggesting that it has the highest antioxidant activity followed by Mentha arvensis (Mint), Murraya koenigii (Curry), Azadirachta indica (Neem) and Emblica officinalis Gaertn (Indian Gooseberry) which is the least significant. For the herbal extract, DPPH, H2O2, culture treatment the values obtained are Ocium sanctum (Tulsi) is significant suggesting that it has high antioxidant activity followed by Murraya koenigii (Curry), Emblica officinalis Gaertn (Indian Gooseberry), Azadirachta indica (Neem) and Mehntha arvensis (Mint) which is the least significant.
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Nutritional evaluation, phytochemical makeup, antibacterial and antioxidant properties of wild plants utilized as food by the Gaddis-a tribal tribe in the Western Himalayas
Aqsa Hafeez
Frontiers in Agronomy
Nowadays, it is generally accepted that medicinal plants play a crucial role in traditional healthcare operations, providing hints to new research fields and in biodiversity protection. However, there is a lack of information on the medicinal uses of plants in many of the interior Himalayan regions. In light of this, the current investigation was initiated in the tribally dominant western Himalayan hinterland. The current study examined five underutilized wild edible plants, namely, Allium rubellum, Berberis chitria, Capsella bursa-pastoris, Stellaria aquatica, and Rheum emodi, for their nutritional qualities, phytochemical analysis, antioxidant activity, and antibacterial activity, which are consumed as food by the Gaddi community of the Bharmour region of the Chamba District, Himachal Pradesh. In this study, the nutritional makeup of these plants was examined in terms of their carbohydrate, protein, sodium, potassium, crude fiber, and fat content. As compared to other investigated...
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Antibacterial and antioxidant activity analysis of some wild medicinal plants
Sahibzada Muhammad Jawad
Journal of entomology and zoology studies, 2017
The present study was performed for the analysis of antimicrobial and antioxidant properties of wild edible plants: Amaranthus thunbergii, Caralluma edulis, Allium astrosanguineum, Rumex patientia and Portulaca oleracea. Which were collected from the arid region of South Waziristan Agency (FATA), Pakistan. The experiment was performed for antibacterial activity for both aqueous and methanolic extracts of selected plants against the three bacteria: Clavibacter michiganensis, Ralstonia solanacearum and Erwinia carotovora. It was observed that the aqueous extract of plants were not effective against these bacteria while the methanolic extract of plants showed light to moderate activity. The antioxidant activities of different fraction of the methanolic extracts were indicated in the range of 69.08-84.89%. From the current study, it may be concluded that the selected plants have the potential of antimicrobial and antioxidant properties, which play a key role in controlling a variety of ...
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Functional foods enhanced with Microbial antioxidants
Charu Gupta
Free radicals plays an important role in the origin of numerous diseases including both lifestyle diseases and physiological diseases like high blood pressure, cancer, diabetes, cardiovascular, neurodegenerative etc. Free radicals act by catalyzing the various toxic oxidative reactions that lead to the formation of toxic lipid peroxides. They also inhibit the enzymes of respiratory chain of mitochondria and damage DNA and proteins which is lethal for the cell. It is therefore important to search for newer alternatives to allopathic medicines with reduced or no side effects. There are numerous medicinal plants that have been reported to possess strong antioxidant activity along with their free radical scavenging activity. Besides plants, various microbes including bacteria and fungi also possess powerful antioxidant activity. Some of the microbes belong to the probiotic group that has the potential to protect the body from the dangerous free radicals. Thus the present paper aims to d...
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Recent Studies on Potential Prebiotic Herbs – A Brief Review
sohani solanke, Nitin Kochar
2021
The impact of prebiotic on gut microbiota has been studied to varying extents, scarcely little importance is given for its effective utilisation to contribute their nutritional and health benefits to cure or manage diseases. Prebiotics compiles non-digestible fibres which serves as the nutrition for gut microbiota. Gut microbiota release biproducts in the intestine which are beneficial for animal as well as human health with different physiological impacts including diabetes, hypertension, obesity, hormonal brain axis, cancer etc. The present review is a comprehensive and an updated compilation of the available works on different plants with prebiotic properties. Due attention has been taken to cover the ongoing trends and recent advances with a perspective vision and their holistic usages and beneficial applications in humans. The current study also shed light on production aspects of prebiotic plants which will enlighten farmers and producers for better economic growth.
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Inhibitory and Bactericidal Potential of Some Indigenous Functional Food-Plants Used in the O.R. Tambo District Municipality of South Africa
Bomkazi Gqaza
Journal of Biosciences and Medicines, 2014
Antimicrobial resistance is a major problem in the management of infectious diseases. African indigenous functional food-plants such as Chenopodium album and Solanum nigrum may constitute important sources of phytochemical constituents for the synthesis of antimicrobial compounds against infectious organisms. The objective of this study was to determine the antimicrobial properties of Chenopodium album and Solanum nigrum-leaves used as functional food-plants in the O.R. Tambo district municipality of South Africa. Organic and aqueous solvent-extracts of C. album and S. nigrum were tested against Staphylococcus aureus (ATCC 29213), Pseudomonas aeruginosa (ATCC127853), Bacillus subtilis (ATCC 6051), Escherichia coli (25922) and Enterococcus faecalis (51299) using standard microbiological techniques. Ciprofloxacin was included in all the experimental runs as positive control antibiotic. The aqueous extracts of both plants were the most active with zones of inhibition diameters ranging from 0 mm-20 mm and minimum inhibitory concentration (MIC50) values ranging from 0.63 mg/mL-10 mg/mL. The positive control antibiotic was highly active with zones of inhibition diameters ranging from 17 mm-31 mm and MIC50 values from 0.0003 mg/mL-0.0005mg/mL for all the bacteria tested. Both extracts were bactericidal with minimum bactericidal concentration (MBC) ranges from 2.5mg/mL-20mg/mL. From the results, it can be concluded that both plants possess compounds with antimicrobial properties, thus validating scientifically their use in traditional medicine. However, more studies to document the respective plant-principles responsible for antimicrobial activity of these plants would shed more light on their functional properties.
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Plant Prebiotics and Their Role in the Amelioration of Diseases
Rachna Verma
Biomolecules, 2021
Prebiotics are either natural or synthetic non-digestible (non-)carbohydrate substances that boost the proliferation of gut microbes. Undigested fructooligosaccharides in the large intestine are utilised by the beneficial microorganisms for the synthesis of short-chain fatty acids for their own growth. Although various food products are now recognized as having prebiotic properties, several others, such as almonds, artichoke, barley, chia seeds, chicory, dandelion greens, flaxseeds, garlic, and oats, are being explored and used as functional foods. Considering the benefits of these prebiotics in mineral absorption, metabolite production, gut microbiota modulation, and in various diseases such as diabetes, allergy, metabolic disorders, and necrotising enterocolitis, increasing attention has been focused on their applications in both food and pharmaceutical industries, although some of these food products are actually used as food supplements. This review aims to highlight the potenti...
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